Experimental Hypothermia Murine Model for Spinal Cord Injury Studies
DOI:
https://doi.org/10.15417/issn.1852-7434.2022.87.1.1420Keywords:
Hypothermia, murines, spinal cordAbstract
Introduction: Given the complexity of hypothermal trial systems in murines, they are expensive. Our objective was to evaluate if the exogenous hypothermal model used in our laboratory for ocular hypothermia was useful for a significant reduction in medullar spine temperature in adult murines.
Materials and Methods: 36 60-day-old adult male Sprague-Dawley rats were used. They were separated into two groups: a normal temperature group at 24 °C (n=18) and a hypothermia group in a cold chamber at 8 °C for 180 minutes (n=18).
Results: The mean rectal temperature was 37.71 ºC ± 0.572 in the normothermia group and 34.03ºC ± 0.250 in the hypothermia group (p <0.0001). The mean medullar temperature was 38.8 ± 0.468 ºC in the normothermia group and 36.4 ± 0.290 ºC in the hypothermia group (p <0.0001).
Conclusion: Using systematic hypothermia in lab rats seems to be promising to evaluate physiologic and pathological mechanisms triggered in the medullar spine. Exposure to cold in the external chamber produces
significant medullar hypothermia in adult rats. Results suggest this might be an adequate and inexpensive medullar hypothermal model.
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